5/30/2023 0 Comments Universal blood typeCharacterization of the Streptococcus pneumoniae BgaC protein as a novel surface beta-galactosidase with specific hydrolysis activity for the gal beta 1-3GlcNAc moiety of oligosaccharides. Inferring pathway activity toward precise disease classification. Extraction of high molecular weight genomic DNA from soils and sediments. Discovery of new glycosidases from metagenomic libraries. Chemoenzymatic synthesis of a type 2 blood group a tetrasaccharide and development of high-throughput assays enables a platform for screening blood group antigen-cleaving enzymes. Symbol nomenclature for graphical representations of glycans. Impacts of papain and neuraminidase enzyme treatment on electrohydrodynamics and IgG-mediated agglutination of type A red blood cells. ABO blood group glycans modulate sialic acid recognition on erythrocytes. The interaction of a carbohydrate-binding module from a Clostridium perfringens N-acetyl-beta-hexosaminidase with its carbohydrate receptor. Structure and metal-dependent mechanism of peptidoglycan deacetylase, a streptococcal virulence factor. The Phyre2 web portal for protein modeling, prediction and analysis. Development of H-specificity in A substance by A-decomposing enzyme from Clostridium tertium A. Destruction of blood group a activity by an enzyme from Clostridium tertium which deacetylates N-acetylgalactosamine in intact blood group substances. Immunological aspects of the acquired B antigen. Identification, molecular cloning and expression of an alpha- N-acetylgalactosaminidase gene from Clostridium perfringens. Crystal structure of alpha-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding. Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Enhancement of biological reactions on cell surfaces via macromolecular crowding. An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. The outer mucus layer hosts a distinct intestinal microbial niche. MetaPathwaysv2.5: quantitative functional, taxonomic and usability improvements. The carbohydrate-active enzymes database (CAZy) in 2013. Mucin glycan foraging in the human gut microbiome. Combination of 16S ribosomal-RNA-targeted oligonucleotide probes with flow-cytometry for analyzing mixed microbial-populations. Metagenomics: Application of genomics to uncultured microorganisms. Toward efficient enzymes for the generation of universal blood through structure-guided directed evolution. A clostridial endo-beta-galactosidase that cleaves both blood group A and B glycotopes. Bacterial glycosidases for the production of universal red blood cells. Abh and related histo-blood group antigens - immunochemical differences in carrier isotypes and their distribution. Transfusion to blood group A and O patients of group B RBCs that have been enzymatically converted to group O. Group-B erythrocytes enzymatically converted to Group-O survive normally in A, B, and O individuals. Goldstein, J., Siviglia, G., Hurst, R., Lenny, L. Modulating the red cell membrane to produce universal/stealth donor red cells suitable for transfusion. The molecular definition of red cell antigens. Their ability to completely convert A to O of the same rhesus type at very low enzyme concentrations in whole blood will simplify their incorporation into blood transfusion practice, broadening blood supply.ĭaniels, G. The galactosaminidase expands activities within the CAZy family GH36. The X-ray structure of the N-acetylgalactosamine deacetylase reveals the active site and mechanism of the founding member of an esterase family. Among the genes encoded in our library of 19,500 expressed fosmids bearing gut bacterial DNA, we identify an enzyme pair from the obligate anaerobe Flavonifractor plautii that work in concert to efficiently convert the A antigen to the H antigen of O type blood, via a galactosamine intermediate. Here we report the functional metagenomic screening of the human gut microbiome for enzymes that can remove the cognate A and B type sugar antigens. Access to efficient enzymes that can convert A and B type red blood cells to ‘universal’ donor O would greatly increase the supply of blood for transfusions.
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